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The International Society for Optical Engineering (1987), vol. 808, p. 126-30
Laser Doppler microscopy of living cytoplasm. Proceedings of the SPIE
Johnson, Dunbar, Corporate Source: Dept. of Plant Sci, Aberdeen Univ., UK
SUMMARY
The use of a laser Doppler microscope, developed to measure flow and/or diffusion in living cells, is described. It analyses Doppler shifts given to the frequency of laser light scattered at a known angle from particles moving in a volume of less than 200 cubic micrometres. This scattering volume is defined in the specimen by an aperture in an image-plane and can be placed within a single cell. Specimens can be vlewed and recorded continuously by video-enhanced differential interference contrast microscopy during experiments so that, independently of laser Doppler measurements, sizes of individual particles in images may be measured and their displacements timed with the aid of a video-micrometer. The authors describe how ways are now needed to constrain and improve computation and analysis of the laser Doppler measurements by incorporating information from the images (21 refs.)